Google Search the Journal web-site:
|
Abstract - Issue Nov 2015, 36 (6) Back
nstantaneous and historical temperature effects on a-pinene
A
novel in vivo β carotene biosensor for heavy metals detection
Ling Shing
Wong,*, Bei Ru Lee, Chia En Koh, Yun Qiu Ong and Chieh Wean Choong
Faculty of
Science, Technology, Engineering and Mathematics, INTI International
University
Persiaran Perdana
BBN, Putra Nilai, 71800 Negeri Sembilan, Malaysia
*Corresponding
Author E-mail: lingshing79@yahoo.com.sg
|
Publication
Data
Paper received:
21 August 2014
Revised received:
14 December 2014
Accepted:
20 March 2015
|
Abstract
A
novel whole cell-based biosensor was constructed using Daucus carota cell as
a biological component, with in vivo β-carotene as a reporter
group. In this biosensor, the cells were immobilized using agarose gel, and
change in in vivo β-carotene, before and after exposure of cells
to heavy metals was noted using spectrophotometer at λ = 450 nm.
Biosensor was found to operate at its optimum condition using cells from
day-14 culture with density of 8 x 105 cells/cuvette, and immobilized with
0.5 % agarose at 45?C. The performance of biosensor was affected by pH with
the best response produced at pH 7.5. Pb and Cu tests showed that biosensor
was able to detect the presence of both heavy metals within the range of 0.01
ppm ? 10.00 ppm. The performance of biosensor decreased in stability test
with prolonged storage of 40 days, with a stable performance obtained after
10 days of storage. The results showed that β-carotene contain in
naturally available cell D. carota responded well to the presence of
heavy metals. It is a good indication that biosensor designed is a good
candidate to be used for environmental toxicity assessment.
Key
words
Biosensor,
β-carotene, Copper, Daucus carota, Lead
|
Copyright
? 2015 Triveni Enterprises. All rights reserved. No part of the Journal can
be reproduced in any form without prior permission. Responsibility
regarding the authenticity of the data, and the acceptability of the
conclusions enforced or derived, rest completely with the author(s).
|
|