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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Nov 2014, 35 (6)                                     Back

nstantaneous and historical temperature effects on a-pinene

Enhanced isolation and culture of highly efficient psychrophilic

oil-degrading bacteria from oil-contaminated soils in South Korea



Van H.T. Pham1, Jaisoo Kim1* and Seung-Woo Jeong2

1Department of Life Science, Graduate School of Kyonggi university, Suwon 443-760, South Korea

2Department of Environmental Engineering, Kunsan National University, Kunsan 573-701, South Korea

*Corresponding Authors Email :



  Publication Data

Paper received:

02 May 2013


Revised received:

28 August 2013


Re-revised received:

25 November 2013



13 January 2014



It is known that isolation of oil-degrading bacterial strains is difficult at low temperatures, and the biodegradation efficiency of oil-contaminated soil is significantly reduced in cold weather. In this study, 14 strains were isolated from oil-contaminated soil that grew well at 10?C by using a newly developed culture method.11 of the 14 isolates were successfully cultured in mineral salts medium containing 1,500 ppm of oil components, 500 ppm each kerosene, gasoline, and diesel as carbon sources, at 10?C for 2 weeks. The oil degradation efficiencies of these 11 isolates ranged from 36% to 100%, as measured by total petroleum hydrocarbon (TPH) degradation analyses. Three strains (Pseudomonas simiae G1-10, P. taiwanensis Y1-4, and P. koreensis Gwa2) displayed complete degradation (100%), and six others (P. frederiksbergensis G2-2, P. arsenicoxydans Y2-1, P. umsongensis Gwa3, P. migulae Gwa5, Rhodococcus jialingiae Y1-1, and R. qingshengii Y2-2) showed relatively high degradation efficiencies (>70%). This study suggests that these isolates can be effectively utilised in thetreatment of oil-contaminated soil in landfarming, especially during winter.


Key words

Oil-contaminated soil, Psychrophilic, Pseudomonas, Rhodococcus, Bioremediation


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