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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Nov 2014, 35 (6)                                     Back

nstantaneous and historical temperature effects on a-pinene

Isolation and identification of antagonistic bacteria from phylloplane of rice as biocontrol agents for sheath blight



Shamima Akter1, Jugah Kadir2*, Abdul Shukor Juraimi3, Halimi Mohd Saud4 and Salha Elmahdi1

1Department of Plant Protection, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia

2Department of Plant Protection, Serdang 43400, Selangor Darul Ehsan, Malaysia

3Department of Crop Science, Serdang 43400, Selangor Darul Ehsan, Malaysia

4Department of Agricultural Technology, Serdang 43400, Selangor Darul Ehsan, Malaysia

*Corresponding Author E-mail: 



Publication Data

Paper received:

25 May 2013


Revised received:

02 December 2013


Re-revised received:

15 December 2013



13 January 2014



A total of 325 bacteria were isolated from both healthy and sheath blight infected leaf samples of rice plants, collected from different places of Malaysia, following dilution technique. Sheath blight pathogen was isolated from infected samples by tissue plating method. Out of 325, 14 isolates were found to be antagonist against the pathogen in pre evaluation test. All the 14 isolates were morphologically characterized. Antagonistic activity of these isolates was further confirmed by adopting the standard dual culture and extracellular metabolite tests. The best isolates were selected, based on the results. In dual culture test, the selected bacterial isolates KMB25, TMB33, PMB38, UMB20 and BMB42 showed 68.44%, 60.89%, 60.22%, 50.00% and 48.22% fungal growth inhibition, respectively and in extracellular metabolite test these bacterial isolates exhibited 93.33%, 84.26%, 69.82%, 67.96% and 39.26% of the same, respectively. Biochemical tests of selected isolates were performed following standard procedure. These bacterial isolates were tentatively identified as fluorescent pseudomonas by morphological and biochemical characterization. The identities were further confirmed by Biolog microstation system as P. fluorescens (UMB20), P. aeruginosa (KMB25, TMB33 and PMB38) and P. asplenii (BMB42) with similarity index ranging from 0.517 to 0.697. The effective bacterial isolates obtained from the present study can be used in the management of soil borne fungal pathogen Rhizoctonia solani, causing sheath blight of rice.


Key words

Bacterial antagonists, Differential characteristics, Oryza sativa, Pseudomonas, Rhizoctonia solani


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