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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Nov 2014, 35 (6)                                     Back

nstantaneous and historical temperature effects on a-pinene

Characterization of sophorolipid biosurfactant produced by Cryptococcus sp. VITGBN2 and its application on Zn(II) removal from electroplating wastewater



Geetanjali Basak and Nilanjana Das*

Environmental Biotechnology Division, School of Biosciences and Technology VIT University, Vellore-632 014, India

*Corresponding Author E-mail: 



Publication Data

Paper received:

19 July 2013


Revised received:

20 October 2013


Re-revised received:

25 December 2013



11 March 2014



The present study aimed at elucidating the role of biosurfactant produced by yeast for the removal of Zn(II) ions from electroplating wastewater. The yeast species isolated from CETP, Vellore, Tamilnadu was identified as Cryptococcus sp.VITGBN2, based on molecular techniques, and was found to be potent producer of biosurfactant in mineral salt media containing vegetable oil as additional carbon source. Chemical structure of the purified biosurfactant was identified as acidic diacetate sophorolipid through GC-MS analysis. Interaction of Zn(II) ions with biosurfactant was monitored using FT-IR, SEM and EDS analysis. Zn (II) removal at 100 mg l-1 concentration was 84.8 % compared were other synthetic surfactants (Tween 80 and sodium dodecyl sulphate), yeast mediated biosurfactant showed enhanced Zn (II) removal in batch mode.? The role of biosurfactant on Zn(II) removal was evaluated in column mode packed with biosurfactant entrapped in sodium alginate beads. At a flow rate of 1 ml min-1 and bed height of 12 cm, immobilized biosurfactant showed 94.34 % Zn(II) removal from electroplating wastewater. The present study confirmed that Zn(II) removal was biosurfactant mediated. This is the first report establishing the involvement of yeast mediated biosurfactant in Zn(II)? removal from wastewater.


Key words

Cryptococcus sp. VITGBN2, Sophorolipid, Zn (II) removal 


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