Journal of Environmental Biology

Comparison of Ecklonia cava, Ecklonia stolonifera and

Eisenia bicyclis for phlorotannin extraction

Muhammad Tanvir Hossain Chowdhury¹, Issa Bangoura¹, Ji-Young Kang¹, Ji Young Cho², Jin Joo³,

Yoo Seong Choi⁴, Dong Soo Hwang⁵ and Yong-Ki Hong¹*

¹Department of Biotechnology, Pukyong National University, Namku, Busan 608-737, Korea

²Department of Marine Biotechnology, Soonchunhyang University, Asan 336-745, Korea

³Department of Applied Chemistry, Kyungpook National University, Bukgu, Daegu 702-701, Korea

⁴Department of Chemical Engineering, Chungnam National University, Yuseonggu, Daejeon 305-764, Korea

⁵Postech Ocean Science & Technology Institute, Pohang University of Science & Technology, Namgu, Pohang 790-784, Korea

*Corresponding Author E-mail: ykhong@pknu.ac.kr

Publication Data

Paper received:

21 January 2013

Revised received:

08 June 2013

Accepted:

31 July 2013

Abstract

Phlorotannins are polyphenols of marine algae, particularly brown seaweed, having multiple biological activities. A reverse phase-high performance liquid chromatography method was developed for rapid and routine quantification of two major phlorotannins, dieckol and phlorofucofuroeckol-A (PFE-A), from boiling water- and organic solvent-extracts of brown seaweeds Ecklonia cava, E. stolonifera and Eisenia bicyclis. The regression equations for dieckol and PFE-A were as follows: the concentration (mg ml-1) = 16.56 ? peak height (cm) + 0.44, and the concentration = 20.60 ? peak height (cm) + 0.11, with correlation coefficients of 0.996 and 0.999, respectively. Compared to organic solvent extraction, the recovery yield of dieckol from boiling water extracts of E. cava, E. stolonifera and E. bicyclis was 86%, 93%, and 98%, respectively. The recovery yield of PFE-A was 74%, 86% and 62%, respectively. Antioxidant activity was detected in each E. bicyclis water extract (91%), followed by E. stolonifera (90%) and E. cava (74%). Dieckol and PFE-A showed almost 9- and 7-fold stronger antioxidant activity than the standard butylhydroxytoluene, and 6- and 4-fold greater than L-ascorbic acid in molar concentration, respectively.

 Key words

Dieckol, Ecklonia cava, Ecklonia stolonifera, Eisenia bicyclis, Phlorofucofuroeckol-A 

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