Journal of Environmental Biology

In vitro studies on effect of different concentration of

NaCl on Jatropha curcas

Sarina Panghal¹* and Shashi S.Soni²

¹Department of Environmental Sciences, M.D. University, Rohtak-124 001, India

²Department of Horticulture, CCSHAU, Hisar-125 004, India

*Corresponding Authors Email : sss84biotech@gmail.com

Publication Data

Paper received:

19 September 2012

Revised received:

30 October 2013

Accepted:

03 December 2013

Abstract

Short-term effect of different concentrations of NaCl on callus cultures of Jatropha curcas was investigated at different concentration of NaCl (0, 20, 40, 60, 80, 100 mM).Results showed a decrease in fresh weight of callus cultures when subjected to increasing concentration of salt in the medium. Callus morphology correspondingly changed from off-white to blackish?brown above 40mM to acutely necrotic stage at 100 mM NaCl .The callus cultures after recurrent selection (at 20mM for 20 days) were transferred to salt free optimized callus regeneration medium expressed 90.0% recovery. The callus placed in 40mM, 60mM concentration of NaCl exhibited moderate tolerance and showed 64.0% and 56.0% recovery. In 80mM concentration, callus showed moderate susceptibility and showed 6.9 % recovery of callus.

 Key words

Necrotic, Recovery, Stress, Susceptibility, Tolerance

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