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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue May 2014, 35 (3)                                     Back

nstantaneous and historical temperature effects on a-pinene

Anti-oxidative enzyme changes associated with chickpea calli

tolerant to Ascochyta rabiei culture filtrate 


Prabhat Kumar1*, M. S. Sangwan2, Naresh Mehta2 and Santosh Kumar3

1Betelvine Research Centre, Islampur, (Bihar Agricultural University, Sabour) Nalanda -801 303, India

2Department of Plant Pathology, CCS Haryana Agricultural University, Hisar-125 004, India

3Jute Research Station, (Bihar Agriculture University, Sabour) Katihar -854 105, India

*Corresponding Authors Email :





Publication Data

Paper received:

24 May 2013


Revised received:

23 September 2013



03 December 2013



Ascochyta blight caused by Ascochyta rabiei (Pass.) Labrousse is a major biotic constraint in production of chickpea. In the present investigation, all chickpea genotypes [E100Y (m), Gaurav, Pb-7 and L550] induced 100% callus on standard medium with greenish colour and fragile structure. These calli were used for in vitro screening against pathogen, A. rabiei culture filtrate at 0, 5, 10, 15 and 20% concentrations. Survival rate of calli in all chickpea's genotypes were reduced significantly at higher concentration (15%) of culture filtrate. The culture filtrate concentration of 20 % was lethal for calli of all chickpea's genotypes. Hence, biochemical changes viz. total soluble proteins and activities of anti-oxidative enzymes (polyphenol oxidase, peroxidase and catalase) were estimated at 15% and below concentration of culture filtrate.? Tolerant calli of resistant genotype, E100 Y (m) revealed significantly higher total soluble proteins (10.04 mg g-1 f.wt. of callus) and activity of anti-oxidative enzymes, polyphenol oxidase (9.0 unit absorbance change min-1 mg-1 protein) and peroxidase (19.09 unit absorbance change min-1 mg-1 protein) and lower catalase (18.65 ? moles of H2O2 utilized min-1 mg-1 protein) at higher (15%) concentration of culture filtrate followed by moderately resistant (Gaurav), and susceptible genotypes (Pb-7 and L550). Thus, higher polyphenol oxidase and peroxidase and lower catalase activity in chickpea's genotypes against culture filtrate of A. rabiei could be used as parameters for screening resistant genotypes to pathogen, A. rabiei.  


 Key words

Anti-oxidative enzymes, Ascochyta rabiei, Callus, Chickpea, Culture filtrate, Soluble proteins 



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