JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Home
    Editor in Chief
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
 
Read Journal
    Current Issue
    Journal Archives
 
For Authors
    Guidelines for Authors
    Terms and Conditions
    Fees and Payments
    Track Paper Status
 
Announcements
    JEB Awards
 

Google Search the Journal web-site:


    Abstract - Issue Mar 2014, 35 (2)                                     Back


nstantaneous and historical temperature effects on a-pinene

Heat stress tolerance in relation to oxidative stress

and antioxidants in Brassica juncea

 

Robin A. Wilson1,2,3, M.K. Sangha1, 2*, S.S. Banga1, A. K. Atwal1 and Shilpa Gupta1

 

1Department of Plant Breeding and Genetics, Punjab Agricultural University, P.O. PAU, Ludhiana-141 004, India

2Department of Biochemistry, Punjab Agricultural University, Ludhiana-141 004, India

3Central Institute of Post Harvest Engineering and Technology, Ludhiana-141 004, India

*Corresponding Author E-mail: manjeet_kaursangha@yahoo.com

 

 

 

Publication Data

Paper received:

16 October 2012

 

Revised received:

29 May 2013

 

Accepted:

31 July 2013

 

Abstract

In the present study fifty genotypes of Brassica juncea were evaluated for heat stress tolerance in terms of biochemical components, in four day old seedlings. Heat shock was given at 45?C for 4.5 hr and thereafter survival percentage, electrolyte leakage and chlorophyll content were estimated. Tolerant genotypes (10) registered survival greater than 65%, moderately tolerant (20) between 35-65% and susceptible (20) less than 35%. Electrolyte leakage was significantly (p<0.001) higher in susceptible genotypes than in tolerant ones with respect to control seedlings. Chlorophyll content showed no significant variation among the tolerant, moderately tolerant and susceptible genotypes, although it registered a decline in response to heat stress. Lipid peroxidation, assessed by malondialdehyde (MDA) in stressed conditions was 4.66 (MDA g-1 f. wt. of tissue) in tolerant genotypes, 7.44 (MDA g-1 f. wt. of tissue) in susceptible genotypes and correlated significantly (r=0.563) with electrolyte leakage. Increase in POD activity under heat stress was maximum in tolerant class with respect to control. CAT activity showed decrease after heat shock treatment in all the three classes but the decrease was 1.3 fold in tolerant genotypes as compared to 1.6 fold in susceptible genotypes. The non-enzymatic antioxidants glutathione and proline registered a significantly (<0.01) high value in tolerant genotypes on heat shock treatment in comparison to susceptible genotypes corroborating the role of antioxidants in mitigating the effect of heat stress in B juncea. The antioxidants and proline seemed to play role in mitigating the effect of heat stress.

 

 

 Key words

Antioxidant relationship, Brassica juncea, Heat stress, Oxidative stress

 

 

Copyright ? 2014 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).