Journal of Environmental Biology

Sensitivity of crude and partially purified acetylcholinesterase from

fish to carbamates and organophosphates

L.G. Tham¹, M.I.E. Halmi¹, M.S.A. Sharif¹, N. Perumal¹, Ghousia Begum², M.A. Syed¹ and M.Y. Shukor^1*

¹Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra,

UPM Serdang, Selangor-43400, Malaysia

²Indian Institute of Chemical Technology, Tarnaka, Hyderabad-500 007, India

*Corresponding Author email : yunus@biotech.upm.edu.my

Publication Data

Paper received:

04 March 2013

Revised received:

07 May 2013

Accepted:

01 June 2013

Abstract

In this work, the selective sensitivity of carbamates and organophosphates (OP) towards crude and partially purified fractions of AChE from the brain of Pangasius pangasius is reported. ANOVA analysis showed that all carbamates tested whether in crude or partially purified fractions showed significant inhibition to AChE activity as compared to control (p<0.05). ANOVA analysis also showed that there was no significant difference between crude and partially purified fraction in terms of inhibition caused by carbamates (p>0.05). Although OPs such as acephate and dimethoate did not show significant inhibition compared to control in the crude fraction, they showed significant inhibition of 8.9 and 9.3% as compared to control (p<0.05) in the partially purified fraction. In addition, although the rest of the OP showed significant inhibition compared to control in both fractions, OPs such as diazinon and trichlorfon showed significantly higher inhibition in the partially purified fraction compared to crude fraction (p<0.05) suggesting that partially purified AChE is more sensitive to OPs compared to the crude fraction. 

 Key words

Carbamates, Organophosphates, AChE, Pangasius pangasius

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