Journal of Environmental Biology

Studies on lipid peroxidation and antioxidant enzymes

in the germinating seeds of Cicer arietinum exposed to cobalt

Barket Ali*

Department of Botany, Govt. College for Women, Srinagar-190 001, India

*Corresponding Author E-mail: barket_ali5@yahoo.com

Publication Data

Paper received:

12 April 2012

Revised received:

16 April 2013

Accepted:

03 June 2013

Abstract

The effect of varying levels of cobalt on per cent germination, proline and protien content, lipid peroxidation, antioxidant enzymes was studied in the germinating seeds of chickpea (Cicer arietinum L.). The surface sterilized seeds, after soaking in 0, 50, 100, 150 or 200 ?M of cobalt for 12 hours, were allowed to germinate in sterilized petri plate at 25 ? 2⁰C, in an incubator. The germinating seeds, at 36 and 72 hrs after germination exhibited a significant decrease in relative water content, mean daily germination, germination percentage, protein content, nitrate reductase activity and antioxidant enzymes (catalase, peroxidase and superoxide dismutase). However, lipid peroxidation and proline level exhibited a linear increase in response to the treatments with passage of time. The seeds showed maximum inhibitory effect at 200 mM Co concentration.

 Key words

Antioxidant enzymes, C. arietinum, Cobalt, Lipid peroxidation 

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