Journal of Environmental Biology

Molecular characterization of Fusarium moniliforme var.

subglutinans isolates

Pradeep Kumar^1,4*, V. K. Gupta², A.K. Misra³ and D. R. Modi⁴

¹The Department of Biotechnology Engineering, Ben Gurion University of The Negev, Be'er Sheva-84105, Israel

²Molecular Glycobiotechnology Group, National University of Ireland Galway-353, Ireland

³Discipline of Biochemistry,Central Institute for Subtropical Horticulture, Lucknow-227 017, India

⁴Department of Biotechnology, BBA University, Lucknow- 226 025, India

*Corresponding Author E-mail: pradeepgkp17@yahoo.co.in

Publication Data

Paper received:

21 July 2012

Revised received:

09 January 2013

Accepted:

27 February 2013

Abstract

Molecular characterization of 42 isolates of mango (Mangifera indica L.) malformation pathogen Fusarium moniliforme var. subglutinans collected from different agro-climatic zones of India were performed using RAPD markers. Twenty RAPD primers of OPA series were selected for screening of which; six primers? OPA-1, 3, 7, 8, 9 and 18 were found to be clearly discriminates and differentiated the F. moniliforme var. subglutinans isolates and showed reproducible banding patterns. A sum total of 598 bands were generated from these random primers having range from 4.0% to 90.9% of similarity among the 42 isolates of F. moniliforme var. subglutinans. The data were used to construct a dendrogram with unweighted pair group method of arithmetical averages (UPGMA) algorithm, using the NTSYS-software. Dendogram revealed more than 90% similarity among all the 42 isolates which shows that all isolates approximately have same genetic identity. Hence, in the current research findings RAPD proved a reproducible and tractable means of differentiating F. moniliforme var. subglutinans isolates.

 Key words

F. moniliforme, RAPD markers, Malformation, Mango, PCR, Variability 

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