Journal of Environmental Biology

Analysis of proline metabolic enzymes in Oryza sativa

under NaCl stress

D.L. Bagdi* and B.P. Shaw

Laboratory of Translational Research and Technology Development, Institute of Life Sciences, Bhubaneswar-751 023, India

*Corresponding Author email : dlbagdi.2000@gmail.com

 Publication Data

?Paper received:

19 December 2011

Revised received:

08 May 2012

Accepted:

26 July 2012

Abstract

The regulation of proline accumulation in seedlings of rice (Oryza sativa L. cv. Badami) was investigated. The increasing concentration of NaCl from 0.5 to 2.5 % progressively increased the proline content in rice. Proline accumulation caused by NaCl was related to protein proteolysis, an increase in OAT, P5CS, P5CR activity, a decrease in PDH activity. The maximum increase in proline content was recorded at 2.5 % NaCl concentration as compared to control and other concentrations of NaCl. The highest significant activity of proline synthesizing enzymes, D¹-Pyrrolline-5-carboxylate synthetase, D¹-Pyrrolline-5-carboxylate reductase and Ornithine-d-aminotransferase with a lowest activity of proline hydrolysis enzymes; Proline dehydrogenase were also recorded at 2.5 % salinity over control and other concentrations of NaCl with a in-significant increase in the activity of D¹-Pyrrolline-5-carboxylate synthetase and Ornithine-d-aminotransferase at 0.5 % concentration of NaCl over control. Externally the addition of 300mg MnCl₂, 220 ml-¹?? strength Hoagland solution, having 1% NaCl, was seen to increase a 893.9 % in proline content of this variety as compared to control.

Key words

Protein proteolysis, NaCl Stress, Proline, Oryza sativa

Copyright ? 2013 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).