Journal of Environmental Biology

Purification of Colocasia esculenta lectin and determination of

its anti-insect potential towards Bactrocera cucurbitae 

 

Kshema Thakur1, Manpreet Kaur2*, Satwinder Kaur3, Amritpal Kaur3,

Sukhdev Singh Kamboj1 and Jatinder Singh1

1Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar-143 005, India

2Department of Human Genetics, Guru Nanak Dev University, Amritsar-143 005, India

3Department of Zoology, Guru Nanak Dev University, Amritsar-143 005, India

*Corresponding Author email : drdhunna@rediffmail.com

 

 

 

 

Publication Data

Paper received:

19 August 2011

 

Revised received:

01 February 2012

 

Accepted:

22 March 2012

 

Abstract

The present study reports the purification of a lectin from Colocasia esculenta (L.) Schott corms and evaluation of its anti-insect potential towards Bactrocera cucurbitae (Coquilett). The lectin was found to be specific towards N-acetyl-D-lactosamine (LacNac), a disaccharide and asialofetuin, a desialylated serum glycoprotein in hemagglutination inhibition assay. Asialofetuin was used as a ligand to purify Colocasia esculenta agglutinin (CEA) by affinity chromatography. The purity of CEA was ascertained by the presence of a single band in reducing SDS-PAGE at pH 8.3. The affinity purified CEA was employed in artificial diet bioassay of second instar larvae (64-72 hr old) of the B. cucurbitae at concentrations ranging between 10-160 µg ml-1. The lectin significantly (p<0.01) decreased the percent pupation and emergence with respect to control. Effect on various enzymes was studied by employing LC50 (51.6 µg ml-1) CEA in the artificial diet bioassay of second instar larvae. All the enzymes tested namely esterases, phosphatases (acid and alkaline), superoxide dismutases, catalase and glutathione-S-transferase showed a significant (p<0.01, p<0.05) increase in their enzyme and specific activities. These results showed that CEA affected normal growth and development and presented stress to the larvae, activating their detoxification and anti-oxidant systems.  Thus, the lectin seems to be a useful candidate for the control measures of B. cucurbitae under the integrated pest management (IPM) system.  

                                                                                       

Key words

Colocasia esculenta, Lectin, Bactrocera cucurbitae, Antioxidant enzymes, Hydrolytic enzymes, IPM

 

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