Journal of Environmental Biology

Analysis of  lactate and malate dehydrogenase enzyme profiles of

selected major carps of  wetland of Calcutta

 

Author Details

 

Madhumita Manna

(Corresponding author)

Department  of  Zoology, Bethune College, Kolkata- 700 006, India.     

e-mail : madhumita.manna09@gmail.com 

Priyanka Chakraborty

Department of Zoology, Bethune College, Kolkata-700 006, India.

 

 

Publication Data

Paper received:

05 January 2011

 

Revised received:

28 May 2011

 

Re Revised received:

27 September 2011

 

Accepted:

18 October 2011

 

Abstract

The East Calcutta Wetland (ECW), a Ramsar site in India, acts as the only sink for both city sewages as well as effluents from the surrounding small- scale industries and is alarmingly polluted with heavy metals. The three best edible major carp species rohu (Labeo rohita,), catla (Catla catla,) and mrigala (Cirrhinus mrigala) were undertaken to monitor  lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) by cellulose acetate electrophoresis (CAE) to assess the effects of pollutants, if any. Crude tissue extracts were prepared from brain, eye, heart, skeletal muscle and kidney tissue respectively from each type of fish. No differences were not found in MDH of catla from both sites for all tissues analyzed in this study. Rohu also showed similar mobility for all tissues except for heart tissue which was distinctly different in fishes from ECW site than that of its counterpart from non ECW site. On the other hand, MDH of two tissues of mrigala, eye and muscle respectively showed different migration patterns. LDH profiles for all tissues of three fish species from both the sites were consistently similar, only the expression levels of muscle LDH of mrigala and kidney LDH of rohu varied little. 

 

Key words

Enzyme profile, East Calcutta wetland, Indian major carps 

 

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