Journal of Environmental Biology
pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
Google Search the Journal web-site:
Abstract - Issue Nov 2011, 32 (6) Back
|
Characterization of two metal resistant Bacillus
strains isolated
from slag disposal site at Author Details |
|
|
|
|
|
|
Department
of Microbiology, |
|
|
Department
of Biophysics, Molecular Biology and Bioinformatics, |
|
(Corresponding
author) |
Department of Botany, e-mail:
tkmbu@yahoo.co.in |
|
Publication Data Paper received: 15
May 2010 Revised
received: 21
September 2010 Accepted: 20 November 2010 |
Abstract Two strains of Bacillus
sp. resistant to arsenate and lead designated as AsSP9 and PbSP6,
respectively were isolated from the slag disposal site. They were identified
to be related to Bacillus cereus cluster on the basis of 16S rDNA based sequence analysis and phenotypic
characteristics. Both were rod-shaped (AsSP9, 2-5 mm
and PbSP6, 2-4 mm), aerobic, salt
tolerant (2-8% NaCl), endospore
forming bacteria with minor differences like the AsSP9 showed sporangial
bulging and PbSP6 had positive lipase activity. The temperature range for
their growth was 20-40oC and pH range 6.0-9.0 with an optimum
temperature of 37oC and pH of 7 for both strains. The principal
nitrogen sources for AsSP9 and PbSP6 were DL-Tryptophan
and L-Phenylalanine, respectively. The suitable carbon source for AsSP9 was
lactose and for PbSP6 sucrose. The heavy metal accumulation efficiency was
found to be 0.0047 mg g-1 of dry mass for AsSP9 and 0.686 mg g-1
of dry mass for PbSP6. Key
words Slag disposal, Bacillus
strains As and Pb resistant, Phylogram |
|
Copyright ? 2011 Triveni Enterprises. All rights reserved. No part
of the Journal can be reproduced in any form without prior permission. Responsibility
regarding the authenticity of the data, and the acceptability of the
conclusions enforced or derived, rest completely with the author(s). |
|