Journal of Environmental BiologypISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP |
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Abstract - Issue Jul 2008, 29 (4) BackIsolation of cadmium-induced DNA sequence in microalga Nannochloropsis
oculata Joong Kee Min2,
Tae Yeon Rhim2, Hyun Woong Shin1 and Mi Young Lee*2 1Department of Marine
Biotechnology, Soonchunhyang University, Asan, Chungnan - 336-600, Korea (Received: March 22, 2007; Revised
received: November 03, 2007; Accepted: December 26, 2007) Abstract: The gene induced in
response to Cd exposure in microalga Nannochloropsis oculata was examined in
this investigation. In order to isolate this gene by using nested PCR,
degenerated primers were designed on the basis of highly conserved regions of
the amino acid sequences of various phytochelatin synthase. The size of the
nested PCR amplification product from Nannochloropsis oculata by using two
degenerated primers was measured to be 266 bp. The 266 bp DNA fragment was
sequenced, and it might be induced by Cd exposure in microalga Nannochloropsis
oculata. Key
words: Cadmium, DNA, Nannochloropsis oculata PDF
of full length paper is available with author (miyoung@sch.ac.kr) Copyright ? 2008
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