Journal of Environmental BiologypISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP |
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Abstract - Issue Jul 2007, 28 (3) BackEffect of Pseudomonas
aeruginosa on the giant freshwater prawn, Macrobrachium rosenbergii
- histopathological and electron microscopic
study K. Ramalingam*1 and 1PG (Aquaculture) and Research Department of Advanced Zoology and
Biotechnology, (Received:
August 04, 2005 ; Revised received: February 10, 2006
; Accepted: March 29, 2006) Abstract: The giant freshwater prawn Macrobrachium rosenbergii was
injected with an inoculum containing LD50
96 hr dose of 107 Pseudomonas aeruginosa (MTCC 1688) to
determine the histopathological effects in vivo. The comparison
of tissues of both the control and the bacterial endotoxin
treated prawns after 96 hr revealed significant degenerative changes in treated
prawns. Both light microscopic and electron microscopic observations revealed
the infiltration of
the tissues of Pseudomonas sp in the muscular and hepatopancreatic tissues of prawn. The muscular tissue
changes in the myofibrillar arrangement with blockage
at the gap junctions and necrotic lesions were observed. The hepatopancreatic cells were vacuolated with hypertrophied
nucleus. Atrophy of hepatopancreatic tubules was
conspicuous. The pathogenicity of Pseudomonas aeruginosa is attributed to its infiltration and
multiplication inside the tissues and the consequent release of extra-cellular
enzymes for its metabolism. The degeneration of host tissues is also attributed
to the latter. Key words: Macrobrachium rosenbergii,
Pseudomonas aeruginosa, Extracellular
proteases, Endotoxin PDF
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